Tris (hydroxymethyl) aminomethane (Tris) yog ib qho tseem ceeb tshuaj reagent siv dav hauv kev sim tshuaj biochemical. Vim nws qhov zoo ntawm purity siab, zoo stability, zoo biocompatibility thiab tsawg toxicity, trimethylolaminomethane tau dav siv nyob rau hauv kev lag luam, molecular biology tshuaj, protein sib cais thiab purification thiab lwm yam teb. Txhawm rau kom ntseeg tau qhov tseeb thiab kev ntseeg siab ntawm cov txiaj ntsig kev sim, nws yog qhov tseem ceeb heev los xaiv txoj hauv kev tsim nyog rau kev tshawb pom zoo thiab kev tswj hwm ntawm trismethylolmethylaminomethane. Cov hauv qab no yuav qhia txog cov txheej txheem tshuaj ntsuam xyuas ntawm tris (hydroxymethyl) aminomethane kom ntxaws, suav nrog cov txheej txheem ntawm lub cev, tshuaj lom neeg thiab chromatographic, thiab lwm yam.
1. Instrumental tsom xam
1. Ion chromatography: Ion chromatography yog ib txoj kev uas feem ntau siv rau kev tsom xam ion, thiab nws kuj tseem siv tau rau kev txiav txim siab ntawm trimethylolaminomethane. Txoj kev no siv cov kab ke los cais cov ions hauv kev daws teeb meem thiab ntsuas cov hom phiaj los ntawm kev txheeb xyuas thaj tsam lossis qhov siab ntawm lub ncov. Ion chromatography yog qhov rhiab heev thiab tsim nyog rau kev tsom xam ntawm siab -purity thiab complex mixs.
2. Gas chromatography: Gas chromatography muaj kev ua tau zoo tshaj plaws hauv kev txheeb xyuas cov organic tebchaw tsis muaj zog. Rau trimethylolaminomethane, nws tsis tshua muaj volatile thiab tsawg haum rau roj chromatography. Txawm li cas los xij, yog tias nws nyob nrog lwm cov tshuaj tsis haum, cov roj chromatography tuaj yeem txiav txim siab rau kev tshuaj xyuas.
2. Chromatography
1. Liquid chromatography: Liquid chromatography yog ib txoj kev analytical feem ntau. Los ntawm kev siv lub siab -kev ua haujlwm ua kua chromatography (HPLC), trismethylol aminomethane tuaj yeem sib cais tau zoo thiab raug txiav txim siab hauv lub sijhawm luv luv. Cov qauv no feem ntau suav tau los ntawm kev ntsuas qhov nqus ntawm tris (hydroxymethyl) aminomethane ntawm ib lub wavelength tshwj xeeb ua ke nrog lub ntsuas UV.
2. Nyias txheej chromatography: Nyias txheej chromatography yog ib txoj kev yooj yim thiab ceev tsom xam. Los ntawm txheej cov qauv ntawm cov txheej nyias nyias chromatographic phaj thiab muab tso rau hauv qhov tsim nyog cov kuab tshuaj, tris -hydroxymethyl aminomethane yuav nce siab nrog cov kuab tshuaj thiab cuam tshuam nrog cov txheej nyias nyias, thiab nws cov ntsiab lus tuaj yeem kwv yees los ntawm kev sib piv qhov kev ncua deb ntawm lub phaj.
3. Spectroscopy
1. Ultraviolet-pom pom kev nqus spectroscopy: Ultraviolet- pom kev nqus spectroscopy yog ib qho kev siv tshuaj molecular absorption spectroscopy. Tris (hydroxymethyl) aminomethane muaj qhov sib txawv ntawm qhov nqus dej hauv thaj tsam UV (tshwj xeeb tshaj yog nyob ntawm 260 nm) thiab tuaj yeem siv los txiav txim siab nws cov concentration, uas tuaj yeem ua kom muaj nuj nqis los ntawm kev sib piv cov absorbance nrog tus qauv nkhaus ntawm kev paub ntau.
2. Nuclear magnetic resonance spectroscopy: nuclear magnetic resonance spectroscopy yog ib txoj hauv kev tsom xam cov qauv, uas muaj superiority nyob rau hauv kev txheeb xyuas thiab purity nrhiav tau ntawm trimethylolaminomethane, thiab tuaj yeem txiav txim siab nws cov qauv molecular thiab ntsuas nws purity los ntawm kev kuaj xyuas lub nuclear magnetic resonance teeb liab ntawm hydrogen, carbonyl methyl lwm yam trimethyl methylmethane hauv cov pa roj carbon dioxide.
4. Lwm txoj kev ntsuas
1. Txoj kev tsom xam dej: trimethylolaminomethane feem ntau yog siv rau hauv daim ntawv ntawm cov tshuaj aqueous, yog li txoj kev ntsuas dej kuj yog ib qho tseem ceeb ntawm kev tshawb nrhiav, thiab cov kev siv dej feem ntau yog txoj kev Karl Fischer.
2. combustion analysis method: Cov txheej txheem combustion tsom xam cov ntsiab lus ntawm cov dej thiab cov pa roj carbon dioxide los ntawm kev hlawv cov tris -hydroxymethylaminomethane qauv thiab ntsuas qhov zoo sib txawv ntawm cov dej thiab carbon dioxide tsim, uas yog tsim nyog rau kev soj ntsuam ntawm cov khoom kuaj.
Cov kev ntsuas sib txawv muaj lawv tus kheej qhov zoo thiab qhov tsis zoo, thiab thaum xaiv tris -hydroxymethylaminomethane txoj kev tsom xam, nws yog ib qho tsim nyog yuav tsum xav txog qhov xwm txheej ntawm tus qauv, kev xav tau purity, lub hom phiaj kev sim thiab lwm yam.
