1. Qhov concentration ntawm qhov tsis
Qhov concentration ntawm TRIS tsis tuaj yeem cuam tshuam qhov ua tau zoo thiab qhov tseeb ntawm cov tshuaj tiv thaiv PCR. Feem ntau, TRIS tsis muaj qhov siab ntawm 10-100 mM yog qhov tsim nyog. Yog tias qhov concentration ntawm qhov tsis muaj tsawg dhau, nws tuaj yeem ua rau tsis ruaj khov pH ntawm cov tshuaj tiv thaiv kab mob, uas tuaj yeem cuam tshuam rau kev ua haujlwm thiab qhov tseeb ntawm cov tshuaj tiv thaiv PCR. Yog tias qhov concentration ntawm qhov tsis yog siab dhau, nws tuaj yeem cuam tshuam cov haujlwm ntawm polymerase, cuam tshuam rau kev ua haujlwm thiab qhov tseeb ntawm PCR cov tshuaj tiv thaiv.
2. Lub pH ntawm qhov tsis
Lub pH ntawm TRIS tsis tuaj yeem cuam tshuam qhov ua tau zoo thiab qhov tseeb ntawm cov tshuaj tiv thaiv PCR. Feem ntau, pH ntawm PCR cov tshuaj tiv thaiv yog nyob nruab nrab ntawm 8.0-8.8. Yog tias pH ntawm qhov tsis muaj qhov tsawg dhau, nws yuav ua rau txo qis hauv kev ua haujlwm ntawm polymerase, uas tuaj yeem cuam tshuam rau kev ua haujlwm thiab qhov tseeb ntawm PCR cov tshuaj tiv thaiv. Yog tias pH ntawm qhov tsis muaj siab dhau lawm, nws yuav ua rau tsis tiav denaturation ntawm DNA qauv, uas tuaj yeem cuam tshuam qhov ua tau zoo thiab qhov tseeb ntawm PCR cov tshuaj tiv thaiv.
3. Lub zog ionic ntawm qhov tsis
Lub zog ionic ntawm TRIS tsis tuaj yeem cuam tshuam qhov ua tau zoo thiab qhov tseeb ntawm cov tshuaj tiv thaiv PCR. Feem ntau, lub zog ionic ntawm TRIS tsis yog nyob nruab nrab ntawm 50-150mM. Yog tias lub zog ionic ntawm qhov tsis muaj tsawg dhau, nws tuaj yeem ua rau tsis ruaj khov ion concentrations hauv cov tshuaj tiv thaiv kab mob, uas tuaj yeem cuam tshuam rau kev ua haujlwm thiab qhov tseeb ntawm PCR cov tshuaj tiv thaiv. Yog tias lub zog ionic ntawm qhov tsis yog siab dhau lawm, nws tuaj yeem cuam tshuam cov haujlwm ntawm polymerase, cuam tshuam rau kev ua haujlwm thiab qhov tseeb ntawm PCR cov tshuaj tiv thaiv.
