Raws li cov cuab yeej tseem ceeb hauv kev tshawb fawb txog lub neej niaj hnub no, cov khoom siv roj ntsha sib xyaw ua ke ntau yam tshuaj reagents, kev npaj enzyme thiab lwm yam khoom siv los ua kom yooj yim cov txheej txheem sim, txhim kho kev ua tau zoo ntawm qhov kev sim thiab ua kom qhov tseeb thiab sib xws ntawm cov txiaj ntsig. Ntawm lawv, kev xaiv cov kab ke tsis yog qhov tseem ceeb heev los tswj kev ruaj ntseg ntawm cov tshuaj tiv thaiv kab mob, tiv thaiv kev ua haujlwm ntawm biomolecules thiab txhim kho cov txheej txheem kev sim. TAPS, qhov tsis zoo ntawm cov roj ntsha tsis zoo, tau dhau los ua cov khoom tseem ceeb hauv ntau cov khoom siv lom neeg vim nws cov khoom siv hluav taws xob thiab cov pH dav.
Ua ntej. Buffering yam ntxwv thiab Scope ntawm kev siv TAPS
TAPS's buffer range yog concentrated ntawm pH 7.7 thiab 9.1, uas yog qhov zoo tshaj pH ib puag ncig rau ntau yam tshuaj lom neeg thiab kev sim molecular biology. Nws qhia tau hais tias muaj peev xwm buffering ruaj khov nyob rau hauv qhov ntau, thiab tuaj yeem tswj cov pH stability ntawm lub cev txawm tias nyob rau hauv lub ntsej muag ntawm ib tug npaum li cas ntawm cov kua qaub - puag ncig. Lub zog qis ionic thiab tsis tshua muaj kev cuam tshuam rau cov qauv thiab kev ua haujlwm ntawm biomolecules ua rau TAPS tshwj xeeb tshaj yog tsim rau kev ua haujlwm uas cuam tshuam txog biomolecules.
Thib ob, kev tiv thaiv ntawm TAPS ntawm cov protein ruaj khov thiab kev ua haujlwm
Cov qauv thiab kev ua haujlwm ntawm cov protein muaj qhov cuam tshuam rau pH hloov pauv, thiab cov khoom muaj zog buffering ntawm TAPS tiv thaiv cov protein denaturation lossis inactivation vim pH hloov. Hauv kev tshawb fawb txog cov protein, TAPS tsis yog feem ntau siv rau kev sib cais ntawm cov protein, kev ua kom huv thiab kev ruaj ntseg, tshwj xeeb tshaj yog nyob rau hauv cov kev sim ua ntawm pH siab dua, qhov uas nws muaj kev cuam tshuam me me rau cov qauv protein thiab kev ua haujlwm. Tsis tas li ntawd, cov khoom zwitterionic ntawm TAPS pab txo qis tsis yog -kev cuam tshuam electrostatic, ntxiv tiv thaiv cov protein los ntawm kev sib sau los yog nag lossis daus.
Thib peb, kev sib raug zoo ntawm TAPS nrog rau lwm cov reagents
Cov khoom siv lom neeg feem ntau muaj ntau yam tshuaj reagents thiab kev npaj enzyme, thiab kev sib raug zoo ntawm cov tshuaj reagents yog qhov tseem ceeb los xyuas kom meej tag nrho cov kev ua tau zoo. TAPS muaj qhov tsis tshua muaj siab rau chelate hlau ions thiab tsis tshua muaj kev cuam tshuam keeb kwm yav dhau los, ua rau nws siv tau nrog cov hlau ion cofactors, enzyme inhibitors, fluorescent probes thiab lwm yam reagents.
Plaub, piv txwv ntawm TAPS kev ua tau zoo txhim kho hauv cov khoom siv lom neeg tshwj xeeb
1. Nucleic acid extraction: TAPS tuaj yeem pab tswj hwm qhov chaw pH tsim nyog thaum lub sij hawm DNA / RNA extraction, zam nucleic acid degradation, thiab xyuas kom muaj kev ruaj ntseg ntawm cov tshuaj tiv thaiv nyob rau hauv cov kauj ruam tom ntej xws li rov qab transcription thiab PCR amplification, yog li txhim kho qhov rhiab heev thiab tshwj xeeb ntawm nucleic acid nrhiav kom tau.
2. Cov khoom siv Electrophoresis: Raws li cov khoom tsis sib xws, TAPS tuaj yeem ua tau raws li cov txheej txheem electrophoresis hauv ntau qhov pH, uas yog qhov tsim nyog rau kev sib cais ntawm cov qauv protein kom raug thiab txhim kho qhov kev daws teeb meem thiab rov ua dua ntawm daim ntawv qhia electrophoresis.
3. Immunoassay: Daim ntawv thov ntawm TAPS nyob rau hauv diluent, ntxuav kua los yog substrate tov yuav optimize antigen-antibody cov tshuaj tiv thaiv tej yam kev mob thiab txo cov uas tsis yog- tshwj xeeb khi, yog li txhim kho qhov raug thiab precision ntawm qhov kev ntsuam xyuas.
